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Nitric Oxide Modulates Vascular Endothelial Growth Factor and Receptors in Chronic Cyclosporine Nephrotoxicity by Fuad S. Shihab; William M. Bennett; Jorge Isaac; Hong Yi; Takeshi F. Andoh is a Medicine article available to read on EtoBox.

What is Nitric Oxide Modulates Vascular Endothelial Growth Factor and Receptors in Chronic Cyclosporine Nephrotoxicity about?

## Background: Vascular endothelial growth factor (vegf) is involved in angiogenesis, wound healing, and inflammation and exerts its effect via tyrosine kinase receptors, fms-like tyrosine kinase (flt-1) and fetal liver kinase (flk-1 or kdr). we have previously shown that vegf is up-regulated in a model of chronic cyclosporine (csa) nephrotoxicity and that l-arginine (l-arg) improved while n-nitro-l-arginine-methyl ester (l-name) worsened fibrosis. we examined the role of nitric oxide modulation on vegf in this model. ## Methods: Pair-fed salt-depleted rats were administered csa, csa + l-name, csa +l-arg, vehicle (vh), vh + l-name or vh +l-arg and were sacrificed at 7 or 28 days. physiologic and histologic changes were studied in addition to the mrna expression of vegf and its receptors flt-1 and kdr/flk-1 by northern blot and the protein expression of vegf by western blot and immunohistochemical staining. ## Results: While l-name worsened renal function and histology, l-arg had the opposite beneficial effect in csa-treated rats. vegf mrna and protein expressions increased with csa, further increased with l-name and became significantly reduced with l-arg. flt-1 expression was simi

Who reads Nitric Oxide Modulates Vascular Endothelial Growth Factor and Receptors in Chronic Cyclosporine Nephrotoxicity?

Author

Fuad S. Shihab; William M. Bennett; Jorge Isaac; Hong Yi; Takeshi F. Andoh

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; ScienceOpen (ISSN 0085-2538)

Published

2003

Language

EN

Field

Medicine (Health Sciences)