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Nitrite Generation and Antioxidant Effects During Neutrophil Apoptosis by Neil L.A. Misso; Craig D. Peacock; D.Neil Watkins; Philip J. Thompson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Nitrite Generation and Antioxidant Effects During Neutrophil Apoptosis about?

Neutrophil apoptosis is important for the resolution of airway inflammation in a number of lung diseases. Inflammatory mediators, endogenous reactive oxygen and nitrogen species, and intracellular and extracellular antioxidants may all influence neutrophil apoptosis. This study investigated the involvement of these factors during apoptosis of neutrophils cultured in vitro. Neutrophils undergoing spontaneous apoptosis in culture as assessed by annexin V binding generated significant amounts of nitrite. Incubation with agonistic anti-Fas monoclonal antibody or tumor necrosis factor-α (TNF-α) enhanced neutrophil apoptosis at 6 h, although it decreased nitrite accumulation. Although granulocyte-macrophage colony-stimulating factor significantly reduced neutrophil apoptosis, this was also associated with decreased nitrite accumulation. In contrast, inhibition of apoptosis at 16 h by dibutyryl cyclic adenosine monophosphate was associated with increased nitrite accumulation. Exogenous glutathione (GSH) or N-acetylcysteine significantly enhanced neutrophil apoptosis at 6 h and stimulated the production of H2O2, which may mediate apoptosis through intracellular hydroxyl radical production.

Who reads Nitrite Generation and Antioxidant Effects During Neutrophil Apoptosis?

Author

Neil L.A. Misso; Craig D. Peacock; D.Neil Watkins; Philip J. Thompson

Publisher

Elsevier BV

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)