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The Microorganisms in Chronically Infected End-stage and Non-end-stage Cystic Fibrosis Patients by Vibeke B. Rudkjøbing; Trine R. Thomsen; Morten Alhede; Kasper N. Kragh; Per H. Nielsen; Ulla R. Johansen; Michael Givskov; Niels Høiby; Thomas Bjarnsholt is a Medicine article available to read on EtoBox.

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Patients suffering from cystic fibrosis (CF) develop chronic lung infections because of highly viscous mucus, where bacteria can form biofilms. In this study, we investigated the microorganisms present in the lungs of end-stage and non-end-stage patients using standard culturing techniques and molecular methods. Tissue and sputum samples (n = 34) from explanted lungs of five end-stage patients were examined along with routine expectorates (n = 15) from 13 patients with non-end-stage CF, representing earlier stages of chronic lung infections. Previously, using peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH), we have shown that Pseudomonas aeruginosa was the sole pathogen in end-stage CF lungs (Pediatr Pulmonol 2009, 44: 547). In this study, this tendency was supported by the results of real-time PCR, confirming previous results obtained by standard culturing and 16S rRNA gene analysis (J Clin Microbiol 2011, 49: 4352). Conversely, the non-end-stage patients were found to harbor several species by culturing. PNA FISH confirmed heterogeneous microbiota and showed that the bacteria were located in monospecies aggregates with no apparent physical interaction between

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Author

Vibeke B. Rudkjøbing; Trine R. Thomsen; Morten Alhede; Kasper N. Kragh; Per H. Nielsen; Ulla R. Johansen; Michael Givskov; Niels Høiby; Thomas Bjarnsholt

Publisher

Oxford University Press (OUP)

Published

2012

Language

EN

Field

Medicine (Life Sciences)