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ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant by Gu, Li-Sha; Tan, Ming-Zhu; Li, Shi-Huan; Zhang, Ting; Zhang, Qi-Qiang; Li, Cheng-Xi; Luo, Xue-Mei; Feng, Jia-Xun; Zhao, Shuai is a Engineering article available to read on EtoBox.

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## Abstract ## Background Application of raw starch-degrading enzymes (RSDEs) in starch processing for biofuel production can effectively reduce energy consumption and processing costs. RSDEs are generally produced by filamentous fungi, such as Penicillium oxalicum, but with very low yields, which seriously hampers industrialization of raw starch processing. Breeding assisted by random mutagenesis is an efficient way to improve fungal enzyme production. ## Results A total of 3532 P. oxalicum colonies were generated after multiple rounds of mutagenesis, by atmospheric and room-temperature plasma (ARTP) and/or ethyl methanesulfonate (EMS). Of these, one mutant A2-13 had the highest RSDE activity of 162.7 U/mL, using raw cassava flour as substrate, a yield increase of 61.1%, compared with that of the starting strain, OXPoxGA15A. RSDE activity of A2-13 further increased to 191.0 U/mL, through optimization of culture conditions. Increased expression of major amylase genes, including the raw starch-degrading glucoamylase gene, PoxGA15A, and its regulatory gene, PoxAmyR, as well as several single-nucleotide polymorphisms in the A2-13 genome, were detected by real-time reverse transcriptio

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Author

Gu, Li-Sha; Tan, Ming-Zhu; Li, Shi-Huan; Zhang, Ting; Zhang, Qi-Qiang; Li, Cheng-Xi; Luo, Xue-Mei; Feng, Jia-Xun; Zhao, Shuai

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central; Springer Science and Business Media LLC (ISSN 1754-6834)

Published

2020

Language

EN

Field

Engineering (Physical Sciences)