Can I read Direct Acridine Orange Fluorescence Examination of Blood Slides Compared to Current Techniques for Malaria Diagnosis on EtoBox?

Direct Acridine Orange Fluorescence Examination of Blood Slides Compared to Current Techniques for Malaria Diagnosis by Frédérick Gay; Boubacar Traoré; Josiane Zanoni; Martin Danis; André Fribourg-Blanc is a Medicine article available to read on EtoBox.

What is Direct Acridine Orange Fluorescence Examination of Blood Slides Compared to Current Techniques for Malaria Diagnosis about?

The renewed interest in the use of fluorochromes for malaria diagnosis prompted us to evaluate the acridine orange fluorescence technique on blood slides, and to compare it with established techniques using thick and thin blood films and the QBC'" malaria test, using the Giemsa-stained thick film technique as our standard method for comparison. We compared 123 positively diagnosed cases and 120 negative cases. For primary samples (day O)? the sensitivity of the thin blood film fluorescence acridine orange technique (AO) was 96.4%, and its specificity was 95.1%. In cases of imported malaria, with a prevalence rate of 16.2%, the positive predictive value was 79.2% and the negative predictive value 99.3%. Sensitivity of A0 was significantly higher than that of Giemsa-stained thin blood films for parasitaemias <5OOO@L. The potential of A0 for species diagnosis of Plasmodium was 852%, using Giemsa-stained thin films as the reference technique. Where QBC'"' imposes a cost limitation, especially in developing countries, despite its high performance, the A0 diagnostic technique is a valuable alternative, because of its simplicity, almost negligible cost, and its diagnostic reliability. The

Who reads Direct Acridine Orange Fluorescence Examination of Blood Slides Compared to Current Techniques for Malaria Diagnosis?

Author

Frédérick Gay; Boubacar Traoré; Josiane Zanoni; Martin Danis; André Fribourg-Blanc

Publisher

Elsevier Science; Oxford University Press; Oxford University Press (OUP); ScienceOpen (ISSN 0035-9203)

Published

1996

Language

EN

Field

Medicine (Health Sciences)