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Cultured vestibular ganglion neurons demonstrate latent HSV1 reactivation by Pamela C. Roehm; Vladimir Camarena; Shruti Nayak; James B. Gardner; Angus Wilson; Ian Mohr; Moses V. Chao is a Medicine article available to read on EtoBox.

What is Cultured vestibular ganglion neurons demonstrate latent HSV1 reactivation about?

## Abstract ## Objectives/Hypothesis: Vestibular neuritis is a common cause of both acute and chronic vestibular dysfunction. Multiple pathologies have been hypothesized to be the causative agent of vestibular neuritis; however, whether herpes simplex type I (HSV1) reactivation occurs within the vestibular ganglion has not been demonstrated previously by experimental evidence. We developed an __in vitro__ system to study HSV1 infection of vestibular ganglion neurons (VGNs) using a cell culture model system. ## Study Design: basic science study. ## Results: Lytic infection of cultured rat VGNs was observed following low viral multiplicity of infection (MOI). Inclusion of acyclovir suppressed lytic replication and allowed latency to be established. Upon removal of acyclovir, latent infection was confirmed with reverse‐transcription polymerase chain reaction and by RNA fluorescent __in situ__ hybridization for the latency‐associated transcript (LAT). A total of 29% cells in latently infected cultures were LAT positive. The lytic ICP27 transcript was not detected by reverse‐transcription polymerase chain reaction (RT‐PCR). Reactivation of HSV1 occurred at a high frequency in latently i

Who reads Cultured vestibular ganglion neurons demonstrate latent HSV1 reactivation?

Author

Pamela C. Roehm; Vladimir Camarena; Shruti Nayak; James B. Gardner; Angus Wilson; Ian Mohr; Moses V. Chao

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0023-852X)

Published

2011

Language

EN

Field

Medicine (Health Sciences)