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Multiphoton Microscopy and Fluorescence Lifetime Imaging - Applications in Biology and Medicine by König, Karsten is a nonfiction available to read on EtoBox.

What is Multiphoton Microscopy and Fluorescence Lifetime Imaging - Applications in Biology and Medicine about?

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. **Contents**Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III:

Who reads Multiphoton Microscopy and Fluorescence Lifetime Imaging - Applications in Biology and Medicine?

Author

König, Karsten

Publisher

Walter De Gruyter Gmbh & Co Kg

Published

2018

Language

EN

ISBN

9783110429985

Category

nonfiction

Subjects

Medical, Science, Technology

Updated

2026-03-24