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Expression of HIF prolyl hydroxylase isozymes in growth plate chondrocytes: Relationship between maturation and apoptotic sensitivity by S.P. Terkhorn; J. Bohensky; I.M. Shapiro; E. Koyama; V. Srinivas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The overall goal of the current study was to examine the functional activity of the prolyl hydroxylases (PHDs) in maturing chondrocytes. Herein, we show for the first time that the PHDs are expressed in the maturing zone of the growth plate, and by a chondrocytic cell line. We determined if this protein and its substrate, hypoxia inducible factor (HIF)‐1α, modulated the induction of apoptosis. Using a chondrocyte cell line that matured in culture, we inhibited HIF‐1α expression using siRNA technology and pharmacologically blocked PHD activity. We noted that PHD suppression sensitized the cells to an apoptotic challenge with H~2~O~2~. We next examined the interplay between the PHDs and HIF‐1α by suppressing HIF‐1α and blocking PHD activity. We noted reduced killing when the mature HIF‐silenced cells were challenged with H~2~O~2~. In contrast, there was limited change in the viability of immature cells. Based on these differences in chondrocyte susceptibility, it is concluded that HIF‐1α sensitizes maturing cells to H~2~O~2~‐mediated killing. We next determined if this change in the viability of the PHD‐inhibited cells was linked to changes in activation of caspase‐3. It

Who reads Expression of HIF prolyl hydroxylase isozymes in growth plate chondrocytes: Relationship between maturation and apoptotic sensitivity?

Author

S.P. Terkhorn; J. Bohensky; I.M. Shapiro; E. Koyama; V. Srinivas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)