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Development of Dried Liposomes Containing β-Galactosidase for the Digestion of Lactose in Milk by Chong-Kook Kim; Hey-Soon Chung; Mi-Kyung Lee; Lee-Na Choi; Moon-Hee Kim is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

What is Development of Dried Liposomes Containing β-Galactosidase for the Digestion of Lactose in Milk about?

The hydrolyzed-lactose milk for lactase-deficient subjects has a sweeter taste than whole milk, and some subjects dislike its taste. In order to cope with this shortcoming, we examined whether b-galactosidase, which hydrolyzes lactose, added to the whole milk in the form of dried liposomes, would be able to digest lactose in milk following the lysis of liposomes in the presence of bile salts. Dried liposomes containing b-galactosidase were prepared in the presence of trehalose by the dehydration-rehydration vesicle method to overcome the instability of the conventional liposome suspension. The stability of liposomal membranes was evaluated by measuring the activity of entrapped b-galactosidase under various storage conditions. By treating liposomes with trehalose, which was found to prevent the fusion of liposomes and the leakage of entrapped drug, the entrapping efficiency increased up to fourfold. Over 95% of dried liposomes which had been stored at 17°C for 60 days were reconstituted to liposomes upon rehydration process. From the stability study, dried liposomes were found to retain 87% of b-galactosidase activity at 17°C after 60 days and to be more stable than the multilamell

Who reads Development of Dried Liposomes Containing β-Galactosidase for the Digestion of Lactose in Milk?

Author

Chong-Kook Kim; Hey-Soon Chung; Mi-Kyung Lee; Lee-Na Choi; Moon-Hee Kim

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)

Published

1999

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)