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Rapid Automated Ion-exchange Analysis of Plasma Tyrosine and Phenylalanine with Data Print-out by I.F. Tarbit; J.P. Richardson; G. Dale is a Chemistry article available to read on EtoBox.

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A rapid automated method with data print-out is described for quantitation of plasma phenylalardne and tyrosine from 0.100 ml of sample. The system uses the Rank-Hilger Chmmaspek amino acid analyser linked to a Digico M16E computer. Amino acid concentrations up to 3000 J& can be quantitated without repeat dilutions and assessment of precision at the 500 rh.f level, produced coefficients of variation of 2.2% for tymsine aad 2.5% for phenylalanine. Recovery determinatians from a plssma poolgave a mean recovery of 99.4% for tyrosine and 99.7% for phenylalanine. Correlation with established Buorimetric $echniques was exee&& (r = 0.986 for &osine, r = 0.976 for phenylalanine). By using the same resin column for both the rapid separation of Qrosine and phenylakmine, and the standard physiological fluid separation, full am&&s capability is retained with easy interchange between the txvo systems.

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Author

I.F. Tarbit; J.P. Richardson; G. Dale

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1980

Language

EN

Field

Chemistry (Physical Sciences)