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Optimization of the Membrane Purification of a Polysaccharide–protein Conjugate Vaccine Using Backpulsing by Francis Meacle; Anne Aunins; Rachel Thornton; Ann Lee is a Engineering article available to read on EtoBox.

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The optimization of a membrane puri®cation step to remove unreacted polysaccharide from a protein±polysaccharide conjugate is described. A key component in this work was the introduction of membrane backpulsing to reduce membrane fouling. The backpulsing parameters of pulse duration, frequency and pressure were optimized to minimize cycle time and buffer usage and to maximize process capacity, while still achieving the required 50-fold puri®cation and greater than 90% step yield. It was determined that optimal performance requires frequent pulses (approximately 1 min À1 ) and short durations (less than 1 s). After implementing the optimal backpulsing conditions, the gradual deterioration in membrane performance which had been observed previously was eliminated and acceptable polysaccharide sieving was maintained throughout the dia®ltration. Depending on the polysaccharide serotype, a 3.5±10-fold reduction in buffer usage and a 3±8-fold reduction in cycle time were accomplished. Interestingly, with the introduction of membrane backpulsing, it was observed that decreasing the shear rate at the membrane signi®cantly increased polysaccharide sieving for backpulsed dia®ltrations.

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Author

Francis Meacle; Anne Aunins; Rachel Thornton; Ann Lee

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0376-7388)

Published

1999

Language

EN

Field

Engineering (Physical Sciences)