About this Chemistry article
Electrochemically assisted Fenton reaction: reaction of hydroxyl radicals with xenobiotics followed by on-line analysis with high-performance liquid chromatography/tandem mass spectrometry by Ulrik Jurva; Håkan V. Wikström; Andries P. Bruins is a Chemistry article available to read on EtoBox.
## Abstract Oxygen radicals are generated __in vivo__ by various processes, often as toxic intermediates in different metabolic transformations, and have been shown to play an important role for a large number of diseases. In this article we introduce an electrochemical flow‐through system that allows generation of hydroxyl radicals for reaction with xenobiotics and subsequent detection of the oxidation products on‐line with high‐performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). The system is based on the Fenton reaction and is predominantly aimed at the generation of hydroxyl radicals; however, by minor variations to the system, a broad range of other radicals can be produced. Optimization of the system was performed with the radical scavenger 5,5‐dimethyl‐1‐pyrroline‐__N__‐oxide (DMPO). Under the same physical conditions, one injection through the electrochemical cell gave a higher yield of the oxidation product __N__‐hydroxy‐5,5‐dimethylpyrrolidin‐2‐one than what was attained after 60 min with a chemical Fenton system catalyzed by ascorbic acid. Since the iron is added as Fe^3+^, the initial mixture is ‘inactive’ until it reaches the electrochemical cell.
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- Author
- Ulrik Jurva; Håkan V. Wikström; Andries P. Bruins
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)