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Nonequivalence of Second Virial Coefficients from Sedimentation Equilibrium and Static Light Scattering Studies of Protein Solutions by Donald J. Winzor; Marcin Deszczynski; Stephen E. Harding; Peter R. Wills is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Nonequivalence of Second Virial Coefficients from Sedimentation Equilibrium and Static Light Scattering Studies of Protein Solutions about?

Experimental data for ovalbumin and lysozyme are presented to highlight the nonequivalence of second virial coefficients obtained for proteins by sedimentation equilibrium and light scattering. Theoretical considerations confirm that the quantity deduced from sedimentation equilibrium distributions is B 22 , the osmotic second virial coefficient describing thermodynamic nonideality arising solely from protein self-interaction. On the other hand, the virial coefficient determined by light scattering is shown to reflect the combined contributions of protein-protein and proteinbuffer interactions to thermodynamic nonideality of the protein solution. Misidentification of the light scattering parameter as B 22 accounts for published reports of negative osmotic second virial coefficients as indicators of conditions conducive to protein crystal growth. Finally, textbook assertions about the equivalence of second virial coefficients obtained by sedimentation equilibrium and light scattering reflect the restriction of consideration to single-solute systems. Although sedimentation equilibrium distributions for buffered protein solutions are, indeed, amenable to interpretation in such terms,

Who reads Nonequivalence of Second Virial Coefficients from Sedimentation Equilibrium and Static Light Scattering Studies of Protein Solutions?

Author

Donald J. Winzor; Marcin Deszczynski; Stephen E. Harding; Peter R. Wills

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)