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Spatiotemporal Protein Crystal Growth Studies Using Microfluidic Silicon Devices by Akira Sanjoh; Tomitake Tsukihara is a Engineering article available to read on EtoBox.

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Fundamental investigations of protein crystallization using miniaturized microfluidic silicon devices were presented towards achieving spatiotemporal nucleation and subsequent post-nucleation growth. The developed microfluidic silicon device was typically composed of crystal growth cell, reservoir cell, and optionally of heater elements for supersaturation control. A specific fine pattern area in the growth cell which was fabricated on the silicon substrate with doped p-and n-type silicon layers, served as spatially selective nucleation site of dissolved protein molecules through electrostatic attractive force. In a model material, hen egg white lysozyme, a large number of crystals were grown on the defined nucleation site evenly spaced from each other, whereas parasitic crystal growth positioned around the selective nucleation site, was suppressed by the effects of electrostatic repulsive force between the doped silicon surface and charged protein molecules. A possible crystallization mechanism of describing the heterogeneous nucleation during the initial stage and during the growth of the crystal at the electrolyte-semiconductor silicon surface is proposed and discussed.

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Author

Akira Sanjoh; Tomitake Tsukihara

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0022-0248)

Published

1999

Language

EN

Field

Engineering (Physical Sciences)