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This chapter outlines methods for engineering three-dimensional fibroblastic cell-derived extracellular matrices (fCDMs) to better replicate in vivo tissue environments. It includes protocols for isolating and culturing primary fibroblastic cells, characterizing these cells, and producing multilayered fCDMs, which are crucial for studying cell-ECM interactions and their implications in health and disease. The chapter emphasizes the importance of using 3D cultures for accurate modeling of fibroblastic behavi
- Author
- Kevin Dzobo
- Language
- EN