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Agrobacterium -Mediated Transient Transformation of Sorghum Leaves for Accelerating Functional Genomics and Genome Editing Studies by Sharma, Rita; Liang, Yan; Lee, Mi Yeon; Pidatala, Venkataramana R.; Mortimer, Jenny C.; Scheller, Henrik V. is a Medicine article available to read on EtoBox.

What is Agrobacterium -Mediated Transient Transformation of Sorghum Leaves for Accelerating Functional Genomics and Genome Editing Studies about?

## Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector constructs is highly desirable. ## Results Here, we report an __Agrobacterium__-mediated transient transformation assay with intact sorghum leaves using green fluorescent protein as marker. It also provides a good monocot alternative to tobacco and protoplast assays with a direct, native and more reliable system for testing single guide RNA (sgRNA) expression construct efficiency. Given the simplicity and ease of transformation, high reproducibility, and ability to test large constructs, this method can be widely adopted to speed up functional genomic and genome editing studies.

Who reads Agrobacterium -Mediated Transient Transformation of Sorghum Leaves for Accelerating Functional Genomics and Genome Editing Studies?

Author

Sharma, Rita; Liang, Yan; Lee, Mi Yeon; Pidatala, Venkataramana R.; Mortimer, Jenny C.; Scheller, Henrik V.

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: Biomed Central, 2008-; Publons; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1756-0500)

Published

2020

Language

EN

Field

Medicine (Health Sciences)