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New potential markers of in vitro tomato morphogenesis identified by mRNA differential display by Anna Torelli; Elisabetta Soragni; Angelo Bolchi; Stefania Petrucco; Simone Ottonello; Camillo Branca is a Agricultural and Biological Sciences article available to read on EtoBox.
What is New potential markers of in vitro tomato morphogenesis identified by mRNA differential display about?
The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such genes and to monitor changes in gene expression accompanying in vitro regeneration, we have adapted the mRNA differential display technique to the comparative analysis of a model system of tomato cotyledons that can be driven selectively toward either shoot or callus formation by means of previously determined growth regulator supplementations. Hormoneindependent transcriptional modulation (mainly down-regulation) has been found to be the most common event, indicating that a non-specific reprogramming of gene expression quantitatively predominates during the early phases of in vitro culture. However, cDNA fragments representative of genes that are either down-regulated or induced in a programme-specific manner could also be identified, and two of them (G35, G36) were further characterized. One of these cDNA fragments, G35, corresponds to an mRNA that is down-regulated muc
Who reads New potential markers of in vitro tomato morphogenesis identified by mRNA differential display?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Anna Torelli; Elisabetta Soragni; Angelo Bolchi; Stefania Petrucco; Simone Ottonello; Camillo Branca
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)
- Published
- 1996
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)