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Boronated protoporphyrin (BOPP): localization in lysosomes of the human glioma cell line SF-767 with uptake modulated by lipoprotein levels by Daniel E Callahan; Trudy M Forte; S.M Javed Afzal; Dennis F Deen; Stephen B Kahl; Kathleen A Bjornstad; William F Bauer; Eleanor A Blakely is a Medicine article available to read on EtoBox.

What is Boronated protoporphyrin (BOPP): localization in lysosomes of the human glioma cell line SF-767 with uptake modulated by lipoprotein levels about?

Purpose: Boronated protoporphyrin (BOPP) is a candidate for use in both boron neutron capture therapy (BNCT) and photodynamic therapy (PDT) of glioblastoma multiforme (GBM). Our objectives are to identify factors that influence the uptake and retention of BOPP in vitro and to determine BOPP distribution in a human glioma cell line in vitro. This information will aid the development of compounds and treatment strategies that increase the effectiveness of BNCT therapy for GBM. Methods and Materials: The amount, distribution pattern, and site of internalization of BOPP were assessed using fluorescence microscopy. Living human glioma (SF-767) cells were imaged after a 24-h exposure to BOPP (20 -135.6 g/ml, normal serum). Dose-dependent uptake of BOPP was determined using both fluorescence microscopy of individual living cells and inductively-coupled plasma-atomic emission spectroscopy (ICP-AES) analysis of cell pellets. Lysosome-or mitochondria-specific fluorescent probes were used to identify the cellular compartment containing BOPP. Two human fibroblast cell lines, AG-1522 (LDL receptor-positive) and GM019-15C (LDL receptor-deficient), were used to investigate LDL receptor-dependent

Who reads Boronated protoporphyrin (BOPP): localization in lysosomes of the human glioma cell line SF-767 with uptake modulated by lipoprotein levels?

Author

Daniel E Callahan; Trudy M Forte; S.M Javed Afzal; Dennis F Deen; Stephen B Kahl; Kathleen A Bjornstad; William F Bauer; Eleanor A Blakely

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)

Published

1999

Language

EN

Field

Medicine (Health Sciences)