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DNA affects the composition of lipoplex protein corona: A proteomics approach by Anna L. Capriotti; Giulio Caracciolo; Giuseppe Caruso; Patrizia Foglia; Daniela Pozzi; Roberto Samperi; Aldo Laganà is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract The distribution of drug delivery systems into the body is affected by plasma proteins adsorbed onto their surface. Furthermore, an exact understanding of the structure and morphology of drug carriers is fundamental to understand their role as gene delivery systems. In this work, the adsorption of human plasma proteins bound to cationic liposomes and to their relative DNA lipoplexes was compared. A shotgun proteomics approach based on HPLC coupled to high resolution MS was used for an efficient identification of proteins adsorbed onto liposome and lipoplex surfaces. The distinct pattern of proteins adsorbed helps to better understand the DNA compaction process. The experimental evidence leads us to hypothesize that polyanionic DNA is associated to the lipoplex surface and can interact with basic plasma proteins. Such a finding is in agreement with recent results showing that lipoplexes are multilamellar DNA/lipid domains partially decorated with DNA at their surface. Proteomics experiments showed that the lipoplex corona is rich of biologically relevant proteins such as fibronectin, histones and complement proteins. Our results provide novel insights to understand how l
Who reads DNA affects the composition of lipoplex protein corona: A proteomics approach?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Anna L. Capriotti; Giulio Caracciolo; Giuseppe Caruso; Patrizia Foglia; Daniela Pozzi; Roberto Samperi; Aldo Laganà
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)