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What is Sanger Sequencing Method Overview about?

Sanger sequencing involves three main steps: 1) chain termination PCR to generate DNA fragments of random lengths, 2) size separation of fragments by gel electrophoresis, and 3) analysis of the gel to determine the DNA sequence. In the first step, DNA polymerase incorporates fluorescently labeled chain-terminating nucleotides during PCR, resulting in fragments of different lengths. In the second step, fragments are separated by size on a gel or capillary. In the third step, the gel or chromatogram is read t

Author

Jane Valine Balino

Language

EN