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Environmental DNA analysis confirms extant populations of the cryptic Irwin’s turtle within its historical range by Cecilia Villacorta-Rath; Thomas Espinoza; Bernie Cockayne; Jason Schaffer; Damien Burrows is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Environmental DNA analysis confirms extant populations of the cryptic Irwin’s turtle within its historical range about?
## Background Approximately 50% of freshwater turtles worldwide are currently threatened by habitat loss, rural development and altered stream flows. Paradoxically, reptiles are understudied organisms, with many species lacking basic geographic distribution and abundance data. The iconic Irwin’s turtle, __Elseya irwini__, belongs to a unique group of Australian endemic freshwater turtles capable of cloacal respiration. Water resource development, increased presence of saltwater crocodiles and its cryptic behaviour, have made sampling for Irwin’s turtle in parts of its range problematic, resulting in no confirmed detections across much of its known range for > 25 years. Here, we used environmental DNA (eDNA) analysis for __E. irwini__ detection along its historical and contemporary distribution in the Burdekin, Bowen and Broken River catchments and tributaries. Five replicate water samples were collected at 37 sites across those three river catchments. Environmental DNA was extracted using a glycogen-aided precipitation method and screened for the presence of __E. irwini__ through an eDNA assay targeting a 127 base pair-long fragment of the NADH dehydrogenase 4 (ND4) mitochondrial g
Who reads Environmental DNA analysis confirms extant populations of the cryptic Irwin’s turtle within its historical range?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Cecilia Villacorta-Rath; Thomas Espinoza; Bernie Cockayne; Jason Schaffer; Damien Burrows
- Publisher
- Springer Science and Business Media LLC
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)