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Can I read Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography on EtoBox?
Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography by Marı́a Sarasa-Nacenta; Yolanda López-Púa; Josep Mallolas; José Luis Blanco; José Ma. Gatell; Xavier Carné is a Chemistry article available to read on EtoBox.
What is Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography about?
A rapid, simple and sensitive high-performance liquid chromatographic (HPLC) assay has been developed for the simultaneous quantification of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma. The method involved the solid-phase extraction of the five drugs and the internal standard (I.S., verapamil) from 400 ml of human plasma. The HPLC analysis used a reversed-phase C analytical column and a mobile phase 18 consisting of a gradient with 15 mM phosphate buffer (pH 5.75)-acetonitrile and UV monitoring. The method was linear over the therapeutic concentration range for the five HIV-protease inhibitors. The accuracy of the method ranged from 98.2 to 106.7% and the precision values ranged from 1.4 to 8.1% for intra-day precision and from 3.1 to 6.4% for the inter-day values.
Who reads Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Marı́a Sarasa-Nacenta; Yolanda López-Púa; Josep Mallolas; José Luis Blanco; José Ma. Gatell; Xavier Carné
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)
- Published
- 2001
- Language
- EN
- Field
- Chemistry (Physical Sciences)