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Transposon Tagging for Gene Discovery by navkir is a document available to read on EtoBox.

Transposon tagging involves using transposable elements as mutagens to tag and isolate unknown genes based on their mutant phenotypes. The first step is to identify a mutant plant with a transposon inserted into a gene of interest. A genomic library is then screened with the transposon sequence to clone the tagged gene. Transposon tagging has been used successfully in plants like maize and snapdragon to isolate genes such as Bz1, P, A1, Cl and C2. Transposons from maize and snapdragon have also been introdu

Author

navkir

Language

EN