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Escherichia Coli—a Model System That Benefits from and Contributes to the Evolution of Proteomics by Pat S. Lee; Kelvin H. Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The large body of knowledge about __Escherichia coli__ makes it a useful model organism for the expression of heterologous proteins. Proteomic studies have helped to elucidate the complex cellular responses of __E. coli__ and facilitated its use in a variety of biotechnology applications. Knowledge of basic cellular processes provides the means for better control of heterologous protein expression. Beyond such important applications, __E. coli__ is an ideal organism for testing new analytical technologies because of the extensive knowledge base available about the organism. For example, improved technology for characterization of unknown proteins using mass spectrometry has made two‐dimensional electrophoresis (2DE) studies more useful and more rewarding, and much of the initial testing of novel protocols is based on well‐studied samples derived from __E. coli__. These techniques have facilitated the construction of more accurate 2DE maps. In this review, we present work that led to the 2DE databases, including a new map based on tandem time‐of‐flight (TOF) mass spectrometry (MS); describe cellular responses relevant to biotechnology applications; and discuss some emerg

Who reads Escherichia Coli—a Model System That Benefits from and Contributes to the Evolution of Proteomics?

Author

Pat S. Lee; Kelvin H. Lee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)