Can I read Fluorescence detected magnetic resonance of the primary donor and inner core antenna chlorophyll in Photosystem I reaction centre protein: Sign inversion and energy transfer on EtoBox?

Fluorescence detected magnetic resonance of the primary donor and inner core antenna chlorophyll in Photosystem I reaction centre protein: Sign inversion and energy transfer by Geoffrey F. W. Searle; Tjeerd J. Schaafsma is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Fluorescence detected magnetic resonance of the primary donor and inner core antenna chlorophyll in Photosystem I reaction centre protein: Sign inversion and energy transfer about?

The Photosystem I reaction centre protein CP1, isolated from barley using polyacrylamide gel electrophoresis showed an EPR (Electron Paramagnetic Resonance) spectrum with the polarisation pattern AEEAAE, typical of the primary donor triplet state 3p700, created via radical pair formation and recombination. 3p700 could also be detected by Fluorescence Detected Magnetic Resonance (FDMR) at hf > 700 nm even in the presence of a large number of chlorophyll antennae. Its zero field splitting parameters, D = 282.5 × 10 -4 cm -1 and E --38.5 x 10 -4 cm -1, were independent of the detection wavelength, and agreed with ADMR (Absorption Detected Magnetic Resonance) and EPR values. The signs of the 3p700 D + E and D -E transitions were positive (increase in fluorescence intensity on applying a resonance microwave field). In contrast, in the emission band 685 < Af < 700 nm FDMR spectra with negative D + E and D-E transitions were detected, and the D value was wavelengthdependent. These FDMR results support an excitation energy transfer model for CP1, derived from time-resolved fluorescence studies, in which two chlorophyll antenna forms are distinguished, with fluorescence at 685 < A t. 700

Who reads Fluorescence detected magnetic resonance of the primary donor and inner core antenna chlorophyll in Photosystem I reaction centre protein: Sign inversion and energy transfer?

Author

Geoffrey F. W. Searle; Tjeerd J. Schaafsma

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0166-8595)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)