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Exportin 1-independent nuclear export of GAPDH by Hans-Dirk Schmitz; Christine Dutiné; Jürgen Bereiter-Hahn is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Exportin 1-independent nuclear export of GAPDH about?

## Abstract Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) is a key enzyme of the glycolytic pathway. Recent studies have demonstrated an additional role in apoptosis: GAPDH is targeted to the nucleus during apoptotic signalling. This nuclear transport has also been observed in serum‐depleted cells, but it is reversible in fibroblasts, in contrast to apoptotic‐induced transport (Eur J Cell Biol 80 (2001) 419). Here, we analyse the serum depletion‐induced transport processes of GAPDH in NIH 3T3 cells. Prolonged serum depletion did not cause cell death, nuclear fragmentation (hoechst staining) or a significant increase in DNA strand‐breaks (comet assay). Using cells expressing green fluorescent protein (GFP)‐tagged GAPDH allowed us to monitor its intracellular localisation by confocal laser scanning microscopy (CLSM). Treatment of cells with the exportin1 inhibitor leptomycin B (LMB) did not influence cytoplasmic localisation of GFP—GAPDH, indicating that nuclear targeting of GAPDH is not constitutive and may be altered via a serum‐dependent regulatory export process. Suprisingly, the export of nuclear GFP—GAPDH after re‐addition of serum to starved cells was not prevented by LMB.

Who reads Exportin 1-independent nuclear export of GAPDH?

Author

Hans-Dirk Schmitz; Christine Dutiné; Jürgen Bereiter-Hahn

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)