Opening book details…
Can I read Metabolism of Olaquindox in Rat Liver Microsomes: Structural Elucidation of Metabolites by High-performance Liquid Chromatography Combined with Ion Trap/time-of-flight Mass Spectrometry on EtoBox?
Metabolism of Olaquindox in Rat Liver Microsomes: Structural Elucidation of Metabolites by High-performance Liquid Chromatography Combined with Ion Trap/time-of-flight Mass Spectrometry by Zhaoying Liu; Lingli Huang; Menghong Dai; Dongmei Chen; Yulian Wang; Yanfei Tao; Zonghui Yuan is a Chemistry article available to read on EtoBox.
What is Metabolism of Olaquindox in Rat Liver Microsomes: Structural Elucidation of Metabolites by High-performance Liquid Chromatography Combined with Ion Trap/time-of-flight Mass Spectrometry about?
## Abstract Olaquindox (__N__‐(2‐hydroxyethyl)‐3‐methyl‐2‐quinoxalincarboxamide‐1,4‐dioxide) is a growth‐promoting feed additive for food‐producing animals. Its toxicity is closely related to the metabolism. The complete metabolic pathways of olaquindox are not revealed. To improve studies of the metabolism and toxicity of olaquindox, its biotransformation in rat liver microsomes and the structure of its metabolites using high‐performance liquid chromatography combined with ion trap/time‐of‐flight mass spectrometry (LC/MS‐ITTOF) were investigated. When olaquindox was incubated with an NADPH‐generating system and rat liver microsomes, ten metabolites (M1–M10) were detected. The structures of these metabolites were identified from mass spectra and comparison of their changes in their accurate molecular masses and fragment ions with those of the parent drug. With the high resolution and good mass accuracy achieved by this technique, the elemental compositions of the metabolites and their fragment ions were exactly determined. The results indicate that the N → O group reduction is the main metabolic pathway of olaquindox metabolism in rat liver microsomes, because abundant 1‐desolaquin
Who reads Metabolism of Olaquindox in Rat Liver Microsomes: Structural Elucidation of Metabolites by High-performance Liquid Chromatography Combined with Ion Trap/time-of-flight Mass Spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Zhaoying Liu; Lingli Huang; Menghong Dai; Dongmei Chen; Yulian Wang; Yanfei Tao; Zonghui Yuan
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2008
- Language
- EN
- Field
- Chemistry (Physical Sciences)