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Can I read In Vivo Gene Transfer by Low-Volume Jet Injection on EtoBox?
In Vivo Gene Transfer by Low-Volume Jet Injection by Régis Cartier; Shuxun V. Ren; Wolfgang Walther; Ulrike Stein; Albert Lewis; Peter M. Schlag; Minglin Li; Priscilla A. Furth is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is In Vivo Gene Transfer by Low-Volume Jet Injection about?
around 7% of chimeric clones assuming that an average full-length ds cDNA contains six RsaI sites (1 of 14; total of 14 ends in seven cDNA fragments). Unlike the 5Ј end cDNA fragment, the 3Ј end fragment has two RsaI blunt ends because the cDNA synthesis primer used in SSH contains a RsaI site. In conclusion, chimeric cDNA clones containing transcripts from different genes are formed during SSH and the subsequent PCR. These chimeric clones usually contain a regenerated RsaI site and can be identified easily by RsaI digestion following PCR amplification of the chimeric cDNA sequences. Acknowledgment. We thank Dr. Billie M. Moats-Staats for critical reading of the manuscript and helpful discussions.
Who reads In Vivo Gene Transfer by Low-Volume Jet Injection?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Régis Cartier; Shuxun V. Ren; Wolfgang Walther; Ulrike Stein; Albert Lewis; Peter M. Schlag; Minglin Li; Priscilla A. Furth
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)