About this Biochemistry, Genetics and Molecular Biology article

Cytoprotective and Antioxidant Effects of Boldine on Tert-butyl Hydroperoxide?induced Damage to Isolated Hepatocytes by R. Bannach; A. Valenzuela; B.K. Cassels; L. J. Núnez-Vergara; H. Speisky is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

Boldine, an aporphine alkaloid, was recently shown by us to exhibit potent antioxidant properties. We report here that boldine concentration-dependently inhibited the peroxidative (accumulation of thiobarbituric acid reactive substances) and lytic damage (trypan blue exclusion and lactate dehydrogenase leakage) to isolated rat hepatocytes induced by tert-butyl hydroperoxide (TBOOH). Boldine (200 gmol/L) fully cytoprotected and completely prevented the peroxidation induced by TBOOH at concentrations equal to or lower than 0.87 mmol/L. However, at a peroxide concentration of 0.91 mmol/L, although boldine completely inhibited lipid peroxidation it largely failed to afford cytoprotection against TBOOH. TBOOH alone (0.83 mmol/L) caused an early (within 60 s) sudden decline of reduced glutathione (by 50%) and an equivalent increase in the levels of oxidized glutathione. Neither of these effects was prevented by the simultaneous addition of a cytoprotective and antioxidant concentration of boldine (200 gmol/L). The delayed addition of boldine to the suspension (after 10 or 20 min), while effectively blocking any further increase in thiobarbituric acid reactive substances, totally failed t

Author

R. Bannach; A. Valenzuela; B.K. Cassels; L. J. Núnez-Vergara; H. Speisky

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0742-2091)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)