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Estimation of Aroma Glycosides of Nutmeg and Their Changes During Radiation Processing by Arul Ananthakumar; Prasad S. Variyar; Arun Sharma is a Chemistry article available to read on EtoBox.

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Glycosidically bound volatile compounds of nutmeg were identified as glyco-conjugates of p-cymene-7-ol, eugenol, methoxyeugenol and ␣terpineol. Using phenyl-␤-glucoside as external standard the contents of these glycosidic precursors were estimated based on the measurement of TLC spot density on a densitometer. p-Cymene-7-ol rutinoside was the major aroma glycoside (3.15 mg/100 g), followed by glucosides of methoxyeugenol (0.61 mg/100 g), eugenol (0.50 mg/100 g) and ␣-terpineol (0.51 mg/100 g). A dose-dependent breakdown of these glycosidic precursors was observed during ␥-radiation processing. Among the four glycosides, ␣-terpineol glucoside was the most sensitive to radiation while p-cymene-7-ol rutinoside was the least sensitive. A reduction in the content of total glycosides by almost 50% was noted at a dose of 5 kGy. Partitioning of aroma glycoside into n-butanol from aqueous extracts was found to result in rapid isolation of aroma glycosides, avoiding time consuming pre-purification on Amberlite XAD-2 column. A routine method based on extraction into n-butanol and subsequent quantification of post-irradiation changes in aroma glycosides on a TLC plate using a densitometer is

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Author

Arul Ananthakumar; Prasad S. Variyar; Arun Sharma

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)