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Development of a Simple and Rapid Latex Test for Rotavirus in Stool Samples by Hideki Kohno; Shiho Akihara; Osamu Nishio; Hiroshi Ushihima is a Medicine article available to read on EtoBox.

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**Abstract** In the fields of infectious diseases, a variety of immunoassay methods has been reported. More simple, rapid and cost‐effective manual tests for infectious diseases are needed. For the purpose of simple diagnosis of rotavirus infection, a rapid test against human rotavirus was developed. As for the preparation of the reagent, clinically isolated human rotavirus was cultured using MA‐104 cells. Then, the cultured virus was purified by an ultracentrifugation method. The purified virus was inactivated with formaldehyde for use as antigen for immunization of rabbits. The antibody was purified and applied to sensitize the surface of latex particles to react with rotavirus in diarrheal stools. The latex agglutination test showed that the sensitivity was 98.2% and the specificity was 94.8%, in comparison with electron microscopy as the gold standard. Furthermore, to measure the virus concentration in stool samples, an automated latex photometric immunoassay system (LPIA) was used, which has been developed for quantitative measurement of agglutination. The range of the reactive viruses detected by the LPIA system was 3`10 ^7^–10 ^9^ virions/mL. The rotavirus‐positive and rotav

Who reads Development of a Simple and Rapid Latex Test for Rotavirus in Stool Samples?

Author

Hideki Kohno; Shiho Akihara; Osamu Nishio; Hiroshi Ushihima

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1328-8067)

Published

2000

Language

EN

Field

Medicine (Health Sciences)