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Adeno-Associated Virus 2-Mediated Intratumoral Prostate Cancer Gene Therapy: Long-Term Maspin Expression Efficiently Suppresses Tumor Growth by Masami Watanabe; Yasutomo Nasu; Yuji Kashiwakura; Norihiro Kusumi; Kenji Tamayose; Atsushi Nagai; Tetsuo Sasano; Takashi Shimada; Hiroyuki Daida; Hiromi Kumon is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Adeno-Associated Virus 2-Mediated Intratumoral Prostate Cancer Gene Therapy: Long-Term Maspin Expression Efficiently Suppresses Tumor Growth about?
Maspin is a member of the serine protease inhibitors and the maspin gene, a tumor suppressor gene, is downregulated in a large fraction of prostate cancers. We evaluated the use of adeno-associated virus (AAV, serotype 2) vector encoding maspin as a means for in vivo gene therapy for human prostate cancer. TUNEL assay of subcutaneously formed LNCaP or DU145 tumors in nude mice showed that intratumoral AAV-mediated maspin expression significantly upregulated the number of apoptotic cells compared with AAV-LacZ treatment. Immunofluorescence double staining for maspin protein and apoptosis in LNCaP tumors showed that the percentage of apoptotic cells in AAV-maspin-mediated maspin-expressing cells was significantly high compared with that in AAV-GFP-mediated GFP-expressing cells. Moreover, significantly fewer CD31-positive microvessels were observed in AAV-maspin-treated tumors compared with the control tumors. These therapeutic responses were highly correlated to persistent maspin expression in tumors, confirmed by Western blot analysis until at least day 56 after treatment. Finally, intratumoral delivery of AAV-maspin significantly suppressed growth of LNCaP and DU145 tumors and impr
Who reads Adeno-Associated Virus 2-Mediated Intratumoral Prostate Cancer Gene Therapy: Long-Term Maspin Expression Efficiently Suppresses Tumor Growth?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Masami Watanabe; Yasutomo Nasu; Yuji Kashiwakura; Norihiro Kusumi; Kenji Tamayose; Atsushi Nagai; Tetsuo Sasano; Takashi Shimada; Hiroyuki Daida; Hiromi Kumon
- Publisher
- Mary Ann Liebert Inc
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)