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The study presents a comparative analysis of six molecular detection methods for the fungal pathogen Magnaporthe oryzae, which causes rice blast disease. Utilizing a unified primer set, the research found that CRISPR-Cas12a-based assays, particularly when combined with LAMP or RPA, demonstrated the highest sensitivity and specificity, detecting down to 1 copy/reaction. This systematic evaluation highlights the potential of CRISPR-based diagnostics for effective field applications in early pathogen detection

Author

Swathi

Language

EN