Can I read Plate-Based Assay for Swarming Motility in Pseudomonas aeruginosa on EtoBox?

Plate-Based Assay for Swarming Motility in Pseudomonas aeruginosa by Dae-Gon Ha; Sherry L. Kuchma; George A. O’Toole is a book available to read on EtoBox.

What is Plate-Based Assay for Swarming Motility in Pseudomonas aeruginosa about?

Swarming motility is one of three distinct modes of motility observed in the gram-negative bacterium Pseudomonas aeruginosa . Swarming motility is defi ned as the movement across a semisolid surface, and in P. aeruginosa requires fl agellar motility and the production of biosurfactants. Swarming motility is thought to occur on gelatinous/viscous surfaces inside a host, such as on epithelial cells. There is currently no standardized in vitro assay to visualize and study swarming motility, and the assays used can vary greatly between laboratory groups. Here, we describe a detailed, reproducible in vitro swarming motility assay for P. aeruginosa . While different protocols have previously been reported in the literature, we hope that adopting this method will improve the reproducibility of these swarming motility assays and allow comparisons of swarming motility fi ndings between and among groups.

Author

Dae-Gon Ha; Sherry L. Kuchma; George A. O’Toole

Publisher

Springer New York

Published

2014

Language

EN

ISBN

9781493904723

Subjects

Science, Biology, Stem