Opening book details…
Can I read Establishment of a modified opsonophagocytic killing assay for anti-pneumococcal surface protein A antibody on EtoBox?
Establishment of a modified opsonophagocytic killing assay for anti-pneumococcal surface protein A antibody by Eisuke Kuroda; Yuka Koizumi; Zhenyu Piao; Hiroki Nakayama; Kazunori Tomono; Kazunori Oishi; Shigeto Hamaguchi; Yukihiro Akeda is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Establishment of a modified opsonophagocytic killing assay for anti-pneumococcal surface protein A antibody about?
## A C T Streptococcus pneumoniae (pneumococcus) is a pathogenic gram-positive bacterium that causes pneumonia, meningitis, and sepsis. Pneumococcal surface protein A (PspA) induces antibodies that protect against lethal infections by pneumococci. PspA is a choline-binding protein present on the cell surface of almost all pneumococcal strains and is a non-capsular polysaccharide vaccine candidate. For research and development of PspAbased vaccines, an in-vitro test system to measure the activity of functional antibodies capable of killing pneumococci is essential. The opsonophagocytic killing (OPK) assay is used to evaluate the opsonic activity of functional antibodies induced by capsular polysaccharide (CPS)-based vaccines (standard OPK assay). Despite the potential of anti-PspA antibodies to protect against lethal infections in mice, the standard OPK assay fails to evaluate anti-PspA antibodies. Using a pneumococcal surface protein C-deficient strain and extending the incubation time of opsonized bacteria, complement, and HL-60 cells reportedly results in enhanced bactericidal activity (modified OPK assay). We aimed to measure the bactericidal activity of anti-PspA antibodies in
Who reads Establishment of a modified opsonophagocytic killing assay for anti-pneumococcal surface protein A antibody?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Eisuke Kuroda; Yuka Koizumi; Zhenyu Piao; Hiroki Nakayama; Kazunori Tomono; Kazunori Oishi; Shigeto Hamaguchi; Yukihiro Akeda
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)