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Impact of Sucrose as Osmolyte on Molecular Dynamics of Mouse Acetylcholinesterase by Lushchekina, Sofya V.; Inidjel, Gaetan; Martinez, Nicolas; Masson, Patrick; Trovaslet-Leroy, Marie; Nachon, Florian; Koza, Michael Marek; Seydel, Tilo; Peters, Judith is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Impact of Sucrose as Osmolyte on Molecular Dynamics of Mouse Acetylcholinesterase about?

The enzyme model, mouse acetylcholinesterase, which exhibits its active site at the bottom of a narrow gorge, was investigated in the presence of different concentrations of sucrose to shed light on the protein and water dynamics in cholinesterases. The study was conducted by incoherent neutron scattering, giving access to molecular dynamics within the time scale of sub-nano to nanoseconds, in comparison with molecular dynamics simulations. With increasing sucrose concentration, we found non-linear effects, e.g., first a decrease in the dynamics at 5 wt% followed by a gain at 10 wt% sucrose. Direct comparisons with simulations permitted us to understand the following findings: at 5 wt%, sugar molecules interact with the protein surface through water molecules and damp the motions to reduce the overall protein mobility, although the motions inside the gorge are enhanced due to water depletion. When going to 10 wt% of sucrose, some water molecules at the protein surface are replaced by sugar molecules. By penetrating the protein surface, they disrupt some of the intra-protein contacts, and induce new ones, creating new pathways for correlated motions, and therefore, increasing the dy

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Author

Lushchekina, Sofya V.; Inidjel, Gaetan; Martinez, Nicolas; Masson, Patrick; Trovaslet-Leroy, Marie; Nachon, Florian; Koza, Michael Marek; Seydel, Tilo; Peters, Judith

Publisher

MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Basel: MDPI AG, 2011- (ISSN 2218-273X)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)