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Differences in in vitro invasive capacity induced by differences in Ki-Ras protein mutations by Fahd Al-Mulla; Elaine M. MacKenzie is a Medicine article available to read on EtoBox.

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## Abstract The p21 proteins encoded by N‐, Ki‐, and H‐__ras__ are small guanine nucleotide‐binding proteins that act as switches in several signal transduction pathways. Recently, evidence has been accumulating to suggest that valine‐12 mutation in the Ki‐Ras protein is associated with lung and colorectal tumours that are more aggressive than those carrying aspartate‐12 mutation. The purpose of this study was to determine whether cells transfected with different Ki‐ras codon‐12 mutants have different biological behaviours __in vitro__ that could reflect the differences in behaviour __in vivo__. For that reason, Rat‐1 fibroblasts transfected with the valine‐12 or aspartate‐12 mutant or the wild‐type Ki‐__ras__ gene were assessed in terms of __in vitro__ invasion, transformation, and VEGF production. Both mutants demonstrated equal abilities to transform Rat‐1 cells and induce VEGF production, while cells transfected with wild‐type Ki‐Ras failed to do so. Most significantly, the valine‐12 mutants demonstrated a greater ability to invade Matrigel than cells expressing the aspartate‐12 mutant or wild‐type Ki‐Ras proteins. This study complements previous experimental data that specific

Who reads Differences in in vitro invasive capacity induced by differences in Ki-Ras protein mutations?

Author

Fahd Al-Mulla; Elaine M. MacKenzie

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

2001

Language

EN

Field

Medicine (Health Sciences)