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Can I read Chemical Synthesis of a Gene for Human Stefin A and its Expression inE. coli on EtoBox?
Chemical Synthesis of a Gene for Human Stefin A and its Expression inE. coli by Michael STRAUSS; Jürgen STOLLWERK; Brigita LENARČIČ; Vito TURK; Klaus-Dieter JANY; Hans Günter GASSEN is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Chemical Synthesis of a Gene for Human Stefin A and its Expression inE. coli about?
A DNA containing the coding sequence for the human cysteine proteinase inhibitor stefin A was obtained by enzymic ligation of chemically synthesized deoxyoligonucleotides, using the Khorana ligation method. The 306-bp synthetic gene carries signals for the initiation and termination of its translation. The gene was expressed in E. coli using a cytoplasmic expression vector and stefin A was secreted under the control of the E. coli alkaline phosphatase signal sequence, respectively. The secreted hybrid protein was shown to exhibit biological properties similar to the native protein isolated from human plasma.
Who reads Chemical Synthesis of a Gene for Human Stefin A and its Expression inE. coli?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Michael STRAUSS; Jürgen STOLLWERK; Brigita LENARČIČ; Vito TURK; Klaus-Dieter JANY; Hans Günter GASSEN
- Published
- 1988
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)