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Validation of a method for simultaneous quantification of total carotenes and tocols in vegetable oils by HPLC by Simone M. Silva; Silvana Aparecida Rocco; Klicia Araújo Sampaio; Thiago Taham; Luiza Helena Meller da Silva; Roberta Ceriani; Antonio J.A. Meirelles is a Agricultural and Biological Sciences article available to read on EtoBox.

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A normal-phase HPLC method for analysis of carotenes, tocopherols and tocotrienols has been developed and validated. In this work we presented a modification to the official AOCS method for analysis of tocols which allowed simultaneous quantification of the three groups of compounds, including carotenes. Analytes were separated using a gradient mobile phase (hexane and isopropanol) and with a gradient flow rate (1-2 mL min À1 ). The column effluent was monitored by Photo Diode Array detector (PDA) set at 292 nm (tocols) and 455 nm (b-carotene) and by fluorescence detector set at an excitation wavelength of 290 and 330 nm emission. Inter-and intra-run accuracies and precision of the analytical method were better than ±15%. The lower limit of quantification was 5.0 mg L À1 for the tocols and 0.1 mg L À1 for carotenes. The method has been applied for the quantification of these compounds in Amazon oils.

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Author

Simone M. Silva; Silvana Aparecida Rocco; Klicia Araújo Sampaio; Thiago Taham; Luiza Helena Meller da Silva; Roberta Ceriani; Antonio J.A. Meirelles

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)

Published

2011

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)