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HPLC‐UV and HPLC‐positive‐ESI‐MS analysis of the diterpenoid fraction from caper spurge ( Euphorbia lathyris ) seed oil by Carlo Bicchi; Giovanni Appendino; Chiara Cordero; Patrizia Rubiolo; Didier Ortelli; Jean‐Luc Veuthey is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Caper spurge (__Euphorbia lathyris__ L.) seed oil contains a series of diterpenoids known as __Euphorbia__ factors, or L‐factors, L1–L9. They are esters of several polyols (lathyrol, epoxylathyrol, hydroxylathyrol and ingenol) and account for about 3–5% of the oil. The percentage of ingenol‐based L‐factors is very low, less than 5% of the diterpenoid fraction, but some of them (factors L5 and L6) are responsible for the irritant and co‐carcinogenic activities of the oil. This paper reports an HPLC‐UV and HPLC‐positive‐ESI‐MS analysis of the diterpenoid fraction of caper spurge seed oil before and after selective hydrolysis of ingenol‐based L‐factors. Separation of lathyrane polyols and esters, and ingenol and its esters was achieved using a chromatographic system consisting of a C~18~ stationary phase and acetonitrile:water as mobile phase. A new macrocyclic constituent, the deoxy __Euphorbia__ factor L1, was identified in the oil. Copyright © 2001 John Wiley & Sons, Ltd.

Who reads HPLC‐UV and HPLC‐positive‐ESI‐MS analysis of the diterpenoid fraction from caper spurge ( Euphorbia lathyris ) seed oil?

Author

Carlo Bicchi; Giovanni Appendino; Chiara Cordero; Patrizia Rubiolo; Didier Ortelli; Jean‐Luc Veuthey

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)