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Anti-Inflammatory Effect of Pineapple Rhizome Bromelain through Downregulation of the NF-B- and MAPKs-Signaling Pathways in Lipopolysaccharide (LPS)-Stimulated RAW264.7 Cells by Orapin Insuan; Phornphimon Janchai; Benchaluk Thongchuai; Rujirek Chaiwongsa; Supaporn Khamchun; Somphot Saoin; Wimonrut Insuan; Peraphan Pothacharoen; Waraporn Apiwatanapiwat; Antika Boondaeng; Pilanee Vaithanomsat is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Bromelain is a mixture of proteolytic enzymes derived from pineapple (Ananas comosus) fruit and stem possessing several beneficial properties, particularly anti-inflammatory activity. However, the molecular mechanisms underlying the anti-inflammatory effects of bromelain are unclear. This study investigated the anti-inflammatory effects and inhibitory molecular mechanisms of crude and purified rhizome bromelains on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells. RAW264.7 cells were pre-treated with various concentrations of crude bromelain (CB) or purified bromelain (PB), and then treated with LPS. The production levels of pro-inflammatory cytokines and mediators, including nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)- were determined by Griess and ELISA assays. The expressions of inducible nitric oxide synthetase (iNOS), cyclooxygenase (COX)-2, nuclear factor kappa B (NF-B), and mitogen-activated protein kinases (MAPKs)-signaling pathway-related proteins were examined by western blot analysis. The pre-treatment of bromelain dose-dependently reduced LPS-induced pro-inflammatory cytokines and mediators, which correlated with dow

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Author

Orapin Insuan; Phornphimon Janchai; Benchaluk Thongchuai; Rujirek Chaiwongsa; Supaporn Khamchun; Somphot Saoin; Wimonrut Insuan; Peraphan Pothacharoen; Waraporn Apiwatanapiwat; Antika Boondaeng; Pilanee Vaithanomsat

Publisher

MDPI AG

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)