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Comparative Evaluation of Different Spectrophotometric Methods for the Determination of Small Amounts of Carboxyhaemoglobin by G. Heinemann; Karin Löschenkohl; H. Schievelbein is a Medicine article available to read on EtoBox.

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Four Spectrophotometric methods measuring HbCO were investigated in comparison with an analyzer (CO-Oximeter) in order to find a method determining HbCO fractions 2 ) below 0.10 with sufficient reliability. The CO-Oximeter was chosen as "reference" method because results of other authors with this instrument yielded good agreement with other methods, e.g. IR-spectrometry, GLC, van Slyke. Only the five wavelength method of Commins & Lawther ((1965) Brit. J. Industr. Med. 22,139-143) can be recommended, for it yielded CV's and sensitivities comparable to the CO-Oximeter. The within-run precision had CVs of 2.3 % to 4.7 %, the sensitivity was 0.0021 to 0.0028. The method is suited even for HbCO measurements of shorter series. ## Vergleichende spektrophotometrische Bestimmung geringer HbCO-Anteile Vier manuelle spektrophotometrische Methoden zur Bestimmung von HbCO wurden im Vergleich zu einem teilmechanisierten Gerät (CO-Oximeter) geprüft, ob mit ihnen Anteile unter 0,10 am Gesamt-Hb HbCO zuverlässig gemessen werden können. Wir wählten das

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Author

G. Heinemann; Karin Löschenkohl; H. Schievelbein

Published

1979

Language

EN

Field

Medicine (Health Sciences)