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Design, synthesis, expression, and characterization of the genes for mouse FcγRIIb1 and FcγRIIb2 cytoplasmic regions by Lixin Chen; Nancy L. Thompson; Gary J. Pielak is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The cytoplasmic regions of the mouse low‐affinity FcγRII isoforms, mFcγRIIb1, and mFcγRIIb2, play a key role in signal transduction by mediating different cellular functions. mFcγRIIb1 has a 94‐residue cytoplasmic region, whereas mFcγRIIb2 has a 47‐residue cytoplasmic region. Genes encoding the cytoplasmic regions of mFcγRIIb1 (b1‐94) and mFcγRIIb2 (b2‐47) were designed, synthesized, and expressed as fusion proteins in __Escherichia coli__. A sequencespecific protease, thrombin, was used to release the b1‐94 peptide, which was purified by using HPLC. The b2‐47 peptide was synthesized chemically. CD spectropolarimetry was employed to examine the secondary structures of b1‐94 and b2‐47. These studies were conducted in aqueous solution, in mixtures of water and trifluoroethanol or methanol, and as a function of temperature. The results indicate that the b1‐94 and b2‐47 structures are sensitive functions of the solvent environment, and that nonaqueous solvents induce significant α‐helical structure.

Who reads Design, synthesis, expression, and characterization of the genes for mouse FcγRIIb1 and FcγRIIb2 cytoplasmic regions?

Author

Lixin Chen; Nancy L. Thompson; Gary J. Pielak

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)