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Formation of the Base Modification 8‐Hydroxyl‐2′ ‐ Deoxyguanosine and DNA Fragmentation Following Seizures Induced by Systemic Kainic Acid in the Rat by Jing Lan; David C. Henshall; Roger P. Simon; Jun Chen is a Neuroscience article available to read on EtoBox.
What is Formation of the Base Modification 8‐Hydroxyl‐2′ ‐ Deoxyguanosine and DNA Fragmentation Following Seizures Induced by Systemic Kainic Acid in the Rat about?
**Abstract:** The formation of oxidative DNA damage as a consequence of seizures remains little explored. We therefore investigated the regional and temporal profile of 8‐hydroxyl‐2′ ‐deoxyguanosine (8‐OHdG) formation, a hallmark of oxidative DNA damage and DNA fragmentation in rat brain following seizures induced by systemic kainic acid (KA). Formation of 8‐OHdG was determined via HPLC with electrochemical detection, and single‐ and double‐stranded DNA breaks were detected using in situ DNA polymerase I‐mediated biotin‐dATP nick‐translation (PANT) and terminal deoxynucleotidyl‐transferase‐mediated nick end‐labeling (TUNEL), respectively. Systemic KA (11 mg/kg) significantly increased levels of 8‐OHdG within the thalamus after 2 h, within the amygdala/piriform cortex after 4 h, and within the hippocampus after 8 h. Levels remained elevated up to sevenfold within these areas for 72 h. Smaller increases in 8‐OHdG levels were also detected within the parietal cortex and striatum. PANT‐positive cells were detected within the thalamus, amygdala/piriform cortex, and hippocampus 24‐72 h following KA injection. TUNEL‐positive cells appeared within the same brain regions and over a similar
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- Author
- Jing Lan; David C. Henshall; Roger P. Simon; Jun Chen
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2001
- Language
- EN
- Field
- Neuroscience (Life Sciences)