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Bellidifolin from Gentianella acuta (Michx.) Hulten protects H9c2 cells from hydrogen peroxide-induced injury via the PI3K- ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Preparation of G. acuta and bellidifolin 2 2.2 LC-MS analysis of G. acuta 2 2.3 Network pharmacology and molecular docking analysis 2 2.4 Reagents 2 2.5 Cell culture and treatment 2 2.6 Establishment of the H2O2-induced injury model 3 2.7 Cell viability assay 3 2.8 Detection of LDH, AST, and CK activities in the culture medium 3 2.9 Detection of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in H9c2 cells 3 2.10 Apoptosis assay 3 2.11 RNA-seq transcriptome analysis 3 2.12 Quantitative reverse transcription PCR (qRT-PCR) 3 2.13 Western blotting 3 2.14 Statistical analysis 3 3 Result 4 3.1 Qualitative analysis of G. acuta via LC-MS 4 3.2 Network pharmacological analysis of G. acuta for the treatment of ischemic heart disease 4 3.3 Molecular docking 4 3.4 Establishment of H2O2-induced cell injury model 5 3.5 Cell viability assay 5 3.6 Effect of bellidifolin on the antioxidant enzyme activities 5 3.7 Bellidifolin protected H9c2 cells from H2O2-induced apoptosis 5 3.8 RNA-seq transcript

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UND

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nonfiction