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Oscillatory Electroosmosis-enhanced Intra/inter-particle Liquid Transport and Its Primary Applications in the Preparative Electrochromatography of Proteins by Zheng Liu; Gang Yin; Shaohua Feng; Donghai Wang; Fuxin Ding; Naiju Yuan is a Chemistry article available to read on EtoBox.

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The concept of generating an oscillatory electroosmotic flux inside the porous particle to enhance the intra-particle mass transport was presented and a new kind of electrochromatography carried out in a five-compartment electrolyzer were developed. The adsorbent was packed in the central compartment, while the neighboring compartments were used as the elution compartments and the electrode compartments, respectively. Chromatographic separations of human serum albumin on Blue Sepharose Fast Flow, bovine serum albumin (BSA) on DEAE-Sepharose Fast Flow, and BSA on hydroxyapatite were carried out, respectively. The adsorption isotherms were shown to be independent of electric field, while the increase in the electric field strength resulted in a linear increase in the magnitude of electroosmotic flux and the improvement of the breakthrough behavior in all cases. The experiment results have demonstrated the effectiveness of the oscillatory electroosmosis in enhancing intra-and inter-particle mass transport and its high potential to large-scale chromatography.

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Author

Zheng Liu; Gang Yin; Shaohua Feng; Donghai Wang; Fuxin Ding; Naiju Yuan

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2001

Language

EN

Field

Chemistry (Physical Sciences)