About this document
1 s2.0 S0003269714002863 Main by deb.basak36 is a document available to read on EtoBox.
This document describes a nonradioactive assay developed to detect DNA repair activity of the Escherichia coli enzyme AlkB, which repairs methylated DNA lesions. The assay utilizes methylated oligonucleotides and a methylation-sensitive restriction enzyme, allowing for rapid detection of AlkB activity without the drawbacks of traditional radioactive methods. The study demonstrates the specificity and efficiency of the assay, paving the way for potential high-throughput screening of AlkB inhibitors.
- Author
- deb.basak36
- Language
- EN