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Characterization of FAMPAC, a newly identified human pancreatic carcinoma cell line with a hereditary background by Sven Eisold; Eduard Ryschich; Michael Linnebacher; Thomas Giese; Dirk Nauheimer; Anja Wild; Detlef K. Bartsch; Markus W. Büchler; Jan Schmidt is a Medicine article available to read on EtoBox.
What is Characterization of FAMPAC, a newly identified human pancreatic carcinoma cell line with a hereditary background about?
## Abstract ## BACKGROUND A novel pancreatic carcinoma cell line, FAMPAC, was identified from investigation of poorly differentiated pancreatic adenocarcinoma cells found in a patient with a familial predisposition to pancreatic carcinoma. A gene responsible for familial pancreatic carcinoma has not been identified to date. ## METHODS The FAMPAC cell line was characterized by its morphology, growth rate, tumorigenicity, and chromosomal analysis. Three known tumor suppressor genes, __p16__/__CDKN2__, __BRCA2__, and __p53__, all of which are important in the development of pancreatic carcinoma and frequently are involved in a variety of cancer syndromes, were analyzed. ## RESULTS FAMPAC cells grew as an adhering monolayer in culture medium supplemented with 10% fetal bovine serum and formed tumors rapidly in nude mice. The doubling time ranged from 24 to 48 hours. Karyotype analysis demonstrated the complexity of chromosomal deletions and rearrangements. The cells were negative for ductal differentiation markers such as cytokeratin 7 and MUC1, indicating poor differentiation. Analysis of FAMPAC cells revealed overexpression of the mutated __p53__ gene (exon 5, codon 175: CGC → CAC),
Who reads Characterization of FAMPAC, a newly identified human pancreatic carcinoma cell line with a hereditary background?
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- Author
- Sven Eisold; Eduard Ryschich; Michael Linnebacher; Thomas Giese; Dirk Nauheimer; Anja Wild; Detlef K. Bartsch; Markus W. Büchler; Jan Schmidt
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0008-543X)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)