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SR13/PMP-22 expression in rat nervous system, in PC12 cells, and C6 glial cell lines by Dr. M. De León; R. L. Nahin; M. E. Mendoza; M. A. Ruda is a Neuroscience article available to read on EtoBox.

What is SR13/PMP-22 expression in rat nervous system, in PC12 cells, and C6 glial cell lines about?

## Abstract SR13/PMP‐22 is a protein that was identified after screening a sciatic nerve cDNA library. Our study focused on comparing the level and pattern of expression of SR13/PMP‐22 protein and RNA. Northern blot analysis revealed that although SR13/PMP‐22 mRNA was present in all nervous tissues and cells studied, levels were at least seven fold higher in the sciatic nerve and the spinal cord. During sciatic nerve postnatal development and maturation, the SR13/PMP‐22 mRNA was detected at 2 days after birth, reached a maximal level at day 24, and decreased to 1/3 of the maximum in adult animals. Nerve transection reduced the level of SR13/PMP‐22 mRNA to less than 5% in the segment distal to the nerve injury. Experiments using in situ hybridization localized the SR13/PMP‐22 mRNA in Schwann cells. Schwann cells present in the vicinity or distal to the nerve cut repressed the signal for the message. In situ hybridization experiments also demonstrated that dorsal root ganglia satellite cells contained the message for SR13/PMP‐22. The SR13/PMP‐22 antisera used in our study showed a complex pattern of staining. As expected, the SR13/PMP‐22 antibody peptide 1 immunoreacted with the scia

Who reads SR13/PMP-22 expression in rat nervous system, in PC12 cells, and C6 glial cell lines?

Author

Dr. M. De León; R. L. Nahin; M. E. Mendoza; M. A. Ruda

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1994

Language

EN

Field

Neuroscience (Life Sciences)