Can I read Insulin-like growth factor-I (IGF-i) stimulates protein synthesis and collagen gene expression in monolayer and lattice cultures of fibroblasts on EtoBox?

Insulin-like growth factor-I (IGF-i) stimulates protein synthesis and collagen gene expression in monolayer and lattice cultures of fibroblasts by Philippe Gillery; Armelle Leperre; François-Xavier Maquart; Jacques-Paul Borel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Insulin-like growth factor-I (IGF-i) stimulates protein synthesis and collagen gene expression in monolayer and lattice cultures of fibroblasts about?

## Abstract Fibroblasts cultivated in three‐dimensional tissue‐like matrices are characterized by a slowed metabolism and a decrease of protein synthesis, unless they are submitted to physical tensions. We checked the effects of insulin like growth factor‐I (IGF‐I), known as a potent stimulator of mitogenesis and protein synthesis for many cell types, in various models of cultures: confluent monolayers, collagen lattices, non‐retracting or retracting fibrin lattices. IGF‐I (1–100 ng · ml^−1^) had no effect on cell divisions in lattice cultures. It was able to stimulate collagen lattice retraction when the medium was supplemented with low concentrations of serum. IGF‐I at 10 or 100 ng · ml^−1^ stimulated collagen and non‐collagen syntheses in all culture systems, but stimulation of collagen synthesis only began at the highest concentration (100 ng · ml^−1^) in retracted lattices. Northern blot and dot‐olot analyses of mRNAs extracted from monolayer cultures of fibroblasts showed that IGF‐I stimulated pro α1(I) collagen synthesis at the pretranslational level. Cycloheximide (7.5 μg · ml^−1^) completely inhibited pro α1(I) collagen gene expression induced by IGF‐I. These results show

Who reads Insulin-like growth factor-I (IGF-i) stimulates protein synthesis and collagen gene expression in monolayer and lattice cultures of fibroblasts?

Author

Philippe Gillery; Armelle Leperre; François-Xavier Maquart; Jacques-Paul Borel

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)